Search for trypsin inhibitor deficient soybean varieties and their genetic studies, Soynean trypsin inhibitors electrophoretic differences among varieties and their fractionation on DEAE-cellulose
전기영동
서793ㄴ
2021-01-30T23:37:12
The protein from soybean seeds were examined by polyacrylamide gel electrophoresis and the trypsin inhibitor(T.I) bands were detected.
The water-extractable protein was fractionated into three fractions by Sephadex G-75 gel filtration. The T.I. activity was found only in the second fraction. Kunitz and Bowman-Birk inhibitors were fractionated by DEAE-cellulose chromatography into seven and six and six fractions, respectively. In kunitz inhibitor, 5 fractions were found to have T,I, activity and 4 fractions in Bowman-Birk inhibitor.
From the band patterns of the protein extracts and those of DEAE-cellulose chromatographic fractions, it was found that band 3 and 4 were T.I. band, corresponding to T<TEX>$$i^1$$</TEX> and T<TEX>$$i^2$$</TEX> band, respectively, In addition, band 6 and 10 were presumed to be T.I. band.
Of the 42 vatieties sampled, 35 revealed only T<TEX>$$i^1$$</TEX> band and 7 only T<TEX>$$i^2$$</TEX> band. The T.I. band patterns were not changed by the culture condition.
The T.I. content, when expressed as the number of trypsin units inhibited(T.U.I), showed remarkable differences from 25 to 76 between varieties. The seedtime and fertilization condition had no effect on the T.I. content. Judged from the results of <TEX>$$F_1$$</TEX> seeds analysis, we assumed that T<TEX>$$i^1$$</TEX> and T<TEX>$$i^2$$</TEX> band were controlled by codominant allele at a single locus.
이석하
대두품종
1
Fractionation
Soybean
26 cm
Trypsin inhibitor 결여 大豆品種의 탐색 및 그의 遺傳育種學的 硏究 / 李弘石, 李錫河,文亢植,羅志英,金秀一
DEAE-cellulose
김수일, 서울대학교 농과대학 농화학과
이홍석, 서울대학교 농과대학 농학과
이석하, 서울대학교 농과대학 농학과
Trypsin inhibitor
나지영
나지영, 서울대학교 농과대학 농화학과
10(1-1)
1985
김수일
대두
Trypsin inhiabitors의 전기영동 검정방법에 의한 대두 품종별 비교 및 DEAE-cellulose에 의한 분리
유전육종학
문항식
품종
Electrophoretic
p. 107-121
문항식, 서울대학교 농과대학 농화학과
Genetic
Soynean trypsin inhibitors electrophoretic differences among varieties and their fractionation on DEAE-cellulose
이홍석
Deficient
검정방법
Varieties
Trypsin inhibitor 결여 大豆品種의 탐색 및 그의 遺傳育種學的 硏究
520.5